Catalog Number - 40031-SP150

40031-SP150 Cogent UDA™ (weak cation exchange, WCX) Semi‑Prep HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering a uniquely powerful mixed‑mode separation mechanism that combines reversed phase, weak cation exchange, and HILIC retention in one stationary phase.

The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This multi‑mode behavior allows highly effective purification of basic, polar, and ionizable compounds, especially in complex matrices where traditional preparative columns often fail to resolve closely related species.

The TYPE‑C silica hydride base provides key performance advantages:

• Faster equilibration between preparative runs

• Reduced sensitivity to mobile phase moisture

• Extremely robust column lifetimes for repeated use

• Minimal salt requirements in HILIC mode (cleaner fractions and MS compatibility)

150 mm Length – High Resolution for Semi‑Prep Purification

The 150 mm length offers enhanced chromatographic resolution for more difficult purification challenges.

• Higher resolution than 50 mm and 100 mm semi‑prep columns

• Improved separation of closely related or co‑eluting compounds

• Increased peak capacity for complex sample matrices

✅ Recommended for purification workflows requiring improved resolution and high purity targets

Flow Rate & System Requirements (10 mm ID, 4 µm)

⚠️ Requires semi‑preparative HPLC system capability

• Typical flow rate range: ~4 – 12 mL/min

• Moderate backpressure for 10 mm ID format

• Designed for higher flow purification workflows

• Compatible with fraction collectors and preparative detectors

✅ Important: A specialized HPLC system is required for columns of this size

✅ Best Practice: Use appropriate sample preparation and a matched guard column to protect the column and maintain long‑term performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Challenging Purifications

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Tunable Selectivity

• WCX mechanism active at pH ≥ 6

• Retention controlled via pH and ionic strength

✅ Ideal when traditional prep C18 or ion exchange columns cannot achieve required purity

TYPE‑C Silica Advantages

Faster Equilibration

• Improves throughput and reduces downtime

No Strict Moisture Control (HILIC)

• Simplifies solvent preparation

Robust Column Lifetimes

• Designed for repeated semi‑prep use

Low Salt Elution (HILIC Mode)

• Cleaner fractions and easier downstream processing

Cogent UDA™ Column Advantages

Mixed‑Mode Flexibility

• RP, HILIC, and WCX in one column

Semi‑Prep Scale Capability (10 mm ID)

• Ideal for purification and scale‑up

High Resolution Performance

• Enhanced separation for difficult compounds

Typical Applications

• Semi‑preparative HPLC purification

• Scale‑up from analytical methods

• Complex matrix separations

• Basic and ionizable compound purification

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires semi‑preparative HPLC system capability

• Designed for higher flow operation (10 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.